AKTIVITAS ANTHELMINTIKA INFUSA DAUN Indigofera tintoria L TERHADAP Haemonchus contortus SECARA IN VITRO
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https://doi.org/10.24843/MIP.2024.V27.i02.p08
Abstrak
Nematoda gastrointestinal adalah satu di antara penyebab berkurangnya efisiensi produksi pada domba dan kambing di Indonesia, terutama parasit abomasum Haemonchus contortus. Parasit ini menyebabkan penyakit Haemonchosis yang menyebabkan anemia dan berakibat fatal bagi domba dan kambing produktif. Penelitian ini bertujuan untuk mengevaluasi daya hambat tetas telur dan mortalitas cacing H. contortus dewasa dari infusa daun Indigofera tintoria L secara in vitro. Pengujian infusa daun Indigofera pada lima konsentrasi yaitu 0% sebagai kontrol negatif, 20%, 40%, 60% dan 80%. Kontrol positif menggunakan Albendazole dengan konsentrasi 2 mg/ ml. Pada konsentrasi tertinggi 80% infusa daun Indigofera mampu menghambat penetasan telur sebesar 95.60% dan memiliki daya mortalitas terhadap cacing H. contortus dewasa sebesar 89.67%. Konsentrasi 80% mempunyai efek yang sama jika dibandingkan dengan kontrol positif yang menggunakan Albendazole. Analisis fitokimia daun Indigofera tintoria L mengandung tanin terkondensasi dan berdaya guna sebagai bioanthelmintika.
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Referensi
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Barrau, E., N. Fabre, I. Fouraste, and H. Hoste. 2005. Effect of bioactive compounds from sainfoin (Onob- rychis viciifolia Scop.) on the in vitro larval migra- tion of Haemonchus contortus: Role of tannins and flavonol glycosides. Parasitology 131: 531–538.
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Eguale, T., D. Tadesse, and M. Giday. 2011. In vitro ant- helmintic activity of crude extracts of five medicinal plants against egg-hatching and larval development of Haemonchus contortus. J. Ethnopharmacol. 98: 319-324.
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Martins, A. C., P. L. F. Bergamasco, G. Felippelli, J. H. Tebaldi, M. F. D. Moraes, A. J. P. Testi, I. M. Lapera, and E. G. L. Hoppe. 2017. Haemonchus contortus resistence to monepantel in sheep: fecal egg count reduction and metabolism of methionine, cystine, and inorganic sulfur in sheep. Br. J. Nutr. 70: 647- 661
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Miller, J. E. and D. W. Haorohov. 2006. Immunilogical aspects of nematode parasite control in sheep. Jo- urnal of Animal Science. 84.E124-E132.
Nawaz, M., S. M. Sajid, J. Zubair, Z. Hussain, Abbasi, A. Mohi-Ud-Din, and M. Waqas. 2014. In vitro and in vivo anthelmintic activity of leaves Azadirachta indica, Dalbergia sisso and Morus alba againts Haemonchus contortus. Global Veterinaria. 13: 996-1001.
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Sakti, A. A., R. W. Kustantinah, R. W. Nurcahyo, L.
Perdani, and M. Ekaningrum. 2018. Extraction of condensed tannins from tropical plants as affected by leaves maturity, maceration time, and centrifugal force. Materials Science Forum. 78 – 84
Sani, R. A., G. D. Gray, and R. L. Baker. 2004. Worm Control for Small Ruminants in Tropical Asia. ACIAR Monograph Series 113. Australian Centre for International Agriculture Research.
Setyono, W. 2014. Kontrol Parasit Menggunakan Hijauan sebagai Agen Anthelmintika pada Kambing Bligon Betina. Tesis. Fakultas Peternakan. Universitas Gadjah Mada. Yogyakarta.
Setyono, W., Kustantinah, E. Indarto, N. D. Dono, Zup- rizal, and I. H. Zulfa. 2019. Calliandra calothyrsus and Artocarpus heterophyllus as anti parasite for Bligon Goat. J. Indonesian Trop. Anim. Agric. 44(4): 400-407.
Alonzo-Diaz, M. A., J. F. J. Torres-Acosta, C. A. Sando- val-Castro, and H. Hoste. 2011. Tannins in tropical tree fodders fed to small ruminants: A friendly foe. Small Rumin. Res. 89: 164-173.
Barrau, E., N. Fabre, I. Fouraste, and H. Hoste. 2005. Effect of bioactive compounds from sainfoin (Onob- rychis viciifolia Scop.) on the in vitro larval migra- tion of Haemonchus contortus: Role of tannins and flavonol glycosides. Parasitology 131: 531–538.
Coles, G. C., C. Bauer, F. H. M. Borgsteede, S. Geerts, T. R. Klei, and M. A. Taylor. 2000. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of ant- helmintic resistance in nematodes of veterinary importance. Vet. Parasitol. 44: 35– 44
Eguale, T., D. Tadesse, and M. Giday. 2011. In vitro ant- helmintic activity of crude extracts of five medicinal plants against egg-hatching and larval development of Haemonchus contortus. J. Ethnopharmacol. 98: 319-324.
Ferreia, L. E., P. M. N. Castro, A. C. S. Changas, S. C. Franca, and R. O. Beleboni. 2013. In vitro ant- helmintic activity of aqueous leaf extract of Annona muricata L (Annonaceae) against Haemonchus contortus from sheep. Experimental Parasitology. 134: 327-332
Gilleard, J. S. 2006. Understanding anthelmintic resis- tance: The need for genomics and genetics. Int. J. Parasitol. 36: 1227-1239
Githiori, J. B. 2004. Evaluation of anthelmintic proper- ties of ethnoveterinary plants preparation used as
livestock dewormers by pastoralist and small holder farmers in Kenya. Doctoral Disertation. Departemen Biomedical Sciences and Veterinary Public Health, SLU. Acta Universitatis Agriculture Science. 76.
Haryuningtyas, D. and W. T. Artama. 2008. Analisis se- kuen Gen Tubulin-β Isotipe 1 Cacing Haemonchus contortus isolat resisten terhadap benzimidazole pada domba di Indonesia. J. AgroBiogen. 4: 45–50
Hoste, H., F. Jackson, S. Athanasiadou, S. M. Thamsborg, and S. O. Hoskin. 2006. The effects of tannin-rich plants on parasitic nematodes in ruminants. Trends Parasitol. 22: 253–261
Jackson, F., M. Varady, and D. J. Bartley. 2012. Mana- ging anthelmintic resistance in goats-can we learn lessons from sheep? Small Rumin. Res. 103: 3–9.
Kaplan, R. M. 2004. Drug resistance in nematodes of veterinary importance: A status report. TRENDS Parasitol. 20: 478–481.
Kustantinah, A., J. Daryatmo, E. R. Orskov, R.W. Mayes, and H. Hartadi. 2010. Utilization of Cassava leaf and Carrica papaya leaf as feed and anthelmintic for goat. Proceedings of the British Society of Animal Science and the Agricultural Research Forum. 1: 114.
Martins, A. C., P. L. F. Bergamasco, G. Felippelli, J. H. Tebaldi, M. F. D. Moraes, A. J. P. Testi, I. M. Lapera, and E. G. L. Hoppe. 2017. Haemonchus contortus resistence to monepantel in sheep: fecal egg count reduction and metabolism of methionine, cystine, and inorganic sulfur in sheep. Br. J. Nutr. 70: 647- 661
Mengist, Z., N. Abeb, G. Gugsa, and N. Kumar. 2014. Assessment of small ruminant Haemonchus and its associated risk factors in and around Finoteselam, Ethiopia. Journal of Agriculture and Veterinary Science. 7(12): 36-41.
Miller, J. E. and D. W. Haorohov. 2006. Immunilogical aspects of nematode parasite control in sheep. Jo- urnal of Animal Science. 84.E124-E132.
Nawaz, M., S. M. Sajid, J. Zubair, Z. Hussain, Abbasi, A. Mohi-Ud-Din, and M. Waqas. 2014. In vitro and in vivo anthelmintic activity of leaves Azadirachta indica, Dalbergia sisso and Morus alba againts Haemonchus contortus. Global Veterinaria. 13: 996-1001.
Ngeh, J. T., W. Jacob, N. Mopoi, and D. Sali. 2007. Ethno veterinary medicine. A practical approach to the treatment of cattle diseases in Sub-Saharan Afri- can. 2nd Edition. Agromisa Foundation and CTA, Wageningen. 88.
Oliveira, L. M. B., C. M. L. Bevilaqua, I. T. F. Macedo, S. M. Morais, M. V. B. Monteiro, C. C. Campello, W. L. C. Ribeiro, and E. F. K. Batista. 2011. Effect of six tropical tanniferous plant extracts on larval exsheathment of Haemonchus contortus. Rev. Bras. Parasitol. Vet. 20: 155–160
Sakti, A. A., R. W. Kustantinah, R. W. Nurcahyo, L.
Perdani, and M. Ekaningrum. 2018. Extraction of condensed tannins from tropical plants as affected by leaves maturity, maceration time, and centrifugal force. Materials Science Forum. 78 – 84
Sani, R. A., G. D. Gray, and R. L. Baker. 2004. Worm Control for Small Ruminants in Tropical Asia. ACIAR Monograph Series 113. Australian Centre for International Agriculture Research.
Setyono, W. 2014. Kontrol Parasit Menggunakan Hijauan sebagai Agen Anthelmintika pada Kambing Bligon Betina. Tesis. Fakultas Peternakan. Universitas Gadjah Mada. Yogyakarta.
Setyono, W., Kustantinah, E. Indarto, N. D. Dono, Zup- rizal, and I. H. Zulfa. 2019. Calliandra calothyrsus and Artocarpus heterophyllus as anti parasite for Bligon Goat. J. Indonesian Trop. Anim. Agric. 44(4): 400-407.
Diterbitkan
2024-04-10
##submission.howToCite##
W., SETYONO et al.
AKTIVITAS ANTHELMINTIKA INFUSA DAUN Indigofera tintoria L TERHADAP Haemonchus contortus SECARA IN VITRO.
Majalah Ilmiah Peternakan, [S.l.], v. 27, n. 2, p. 105-109, apr. 2024.
ISSN 2656-8373.
Tersedia pada: <https://ojs.unud.ac.id./index.php/mip/article/view/120931>. Tanggal Akses: 22 apr. 2025
doi: https://doi.org/10.24843/MIP.2024.V27.i02.p08.
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