Staphylococcus aureus Resistan terhadap Berbagai Antimikroba yang Diisolasi dari Susu Sapi di Indonesia
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https://doi.org/10.24843/bulvet.2023.v15.i02.p20
Abstrak
Staphylococcus aureus (S. aureus) merupakan salah satu penyebab utama mastitis pada sapi di seluruh dunia. Penggunaan antimikroba secara tidak tepat di peternakan sapi menyebabkan peningkatan bakteri yang resistan terhadap antimikroba. Penelitian ini bertujuan untuk menginvestigasi profil resistansi antimikroba S. aureus yang diisolasi dari susu sapi. Dua belas isolat S. aureus yang diisiolasi dari sampel susu sapi di Bogor, Indonesia, diuji terhadap tujuh antimikroba menggunakan metode agar difusi menurut Kirby-Bauer. Hasil penelitian menunjukkan bahwa 58.3 % isolat merupakan S. aureus resistan terhadap beberapa antimikroba. Isolat S. aureus yang diuji resistan terhadap penisilin G (66,7 %), ampisilin (66,7 %), vankomisin (58,3 %), dan basitrasin (58,3 %). Sebagian besar isolat sensitif terhadap siprofloksasin (91,7 %), gentamisin (66,7 %), dan kloramfenikol (66,67 %). Pengobatan mastitis harus mempertimbangkan resistansi antimikroba dari bakteri penyebab penyakit.
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Referensi
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Chaalal W, Aggad W, Zidane K, Saidi N, Kihal M. 2016. Antimicrobial susceptibility profiling of Staphylococcus aureus isolates from milk. Braz. Microbiol. Res. J. 13(3): 1-7.
[CLSI]. Clinical and Laboratory Standards Institute. 2020. Performance standards for antimicrobial disk and dilution susceptibility test for bacteria isolated from animals. 5th ed. CLSI supplement VET01S. The USA.
Daka D, Silassie SG, Yihdego D. 2012. Antibiotic-resistance Staphylococcus aureus isolated from cow’s milk in the Hawassa area, South Ethiopia. Ann. Clin. Microbiol. Antimicrob. 11(26): 1-6.
Devriese LA, Hommez J. 1975. Epidemiology of methicillin-resistant Staphylococcus aureus in dairy herds. Res. Vet. Sci. 19: 23 -7
[FDA] Food and Drug Administration. 2005. Isolation and identification of Staphylococcus aureus in foods. In Bacteriological analytical manual 8th ed. Gaithersbug: AOAC International.
Fessler A, Scott C, Kadlec K, Ehricht R, Monecke S, Schwarz S. 2010. Characterization of methicillin-resistant Staphylococcus aureus ST398 from cases of bovine mastitis. J. Antimicrob. Chem. 65(4): 619-625.
Kateete DP, Kabugo U, Baluku H, Nyakarahuka L, Kyobe S, Okee M, Najjuka CF, Joloba ML. 2013. Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda. PlosOne. 8(5): 1-12.
Khairullah AR, Ramandinianto SC, Effendi MH. 2020. A Review of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) on Bovine Mastitis. Sys. Rev. Pharm. 11(7): 172-183.
Klobongona MLMNB, Afiff U, Rotinsulu DA. 2019. Efektivitas antimikroba terhadap Pasteurella multocida dan Mannheimia haemolityca dari sapi yang diduga menderita bovine respiratory disease kompleks. ARSHI Vet. Lett. 3(2): 39-40.
Li JP, Zhou HJ, Yuan L, He T, Hu SH. 2009. Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China. J. Zhejiang Univ. Sci. B. 10(10): 753-760.
Meutia N, Rizalsyah, Saiful R, Mitha KS. 2016. Residu antibiotik dalam air susu segar yang berasal dari peternakan di wilayah Aceh Besar. JITV. 16(1): 1-5.
Nam HM, Lee AL, Jung SC, Kim MN, Jang GC, Wee SH, Lim SK. 2011. Antimicrobial susceptibility of Staphylococcus aureus and characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Korea. Foodborne Pathog. Dis. 8(2): 231-238.
Nurhayati IS, Martindah. 2015. Pengendalian mastitis subklinis melalui pemberian antibiotik saat periode kering pada sapi perah. Wartazoa. 25(2): 65-74.
Ruegg PL. 2017. A 100-year review: mastitis detection, management, and prevention. J. Dairy Sci. 100(12): 10381-10397.
Rotinsulu DA, Afiff U, Septiriyanti D. 2022. Resistansi Escherichia coli asal feses sapi di wilayah Bogor terhadap antimikrob. ARSHI Vet. Lett. 6(4): 75-76.
Saini V, McClure JT, Scholl DT, DeVries TJ, Barkema HW. 2012. Herd-level association between antimicrobial use and antimicrobial resistance in bovine mastitis Staphylococcus aureus isolates on Canadian dairy farms. J. Dairy Sci. 95(4): 1921-1929.
Sashidaran S, Prema B, Yoga LL. 2011. Antimicrobial drug resistance of Staphylococcus aureus in dairy products. Asian Pac. J. Trop. Biomed. 1(2): 130-132.
Tyasningsih W, Ramandinianto SC, Ansharieta R, Witaningrum AM, Permatasari DA, Wardhana DK, Effendi MH, Ugbo EN. 2022. Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia, Vet. World. 15(8): 2021-2028.
Wang K, Cha J, Liu K, Deng J, Yang B, Xu H, Wang J, Zhang L, Gu X, Huang C, Qu W. 2022. The prevalence of bovine mastitis-associated Staphylococcus aureus in China and its antimicrobial resistance rate: A meta-analysis. Front. Vet. Sci. 10(9): 1006676.
Carter GR, Cole JR. 1990. Diagnostic Procedures in Veterinary Bacteriology and Mycology. San Diego (US): Academic Press. Inc.
Chaalal W, Aggad W, Zidane K, Saidi N, Kihal M. 2016. Antimicrobial susceptibility profiling of Staphylococcus aureus isolates from milk. Braz. Microbiol. Res. J. 13(3): 1-7.
[CLSI]. Clinical and Laboratory Standards Institute. 2020. Performance standards for antimicrobial disk and dilution susceptibility test for bacteria isolated from animals. 5th ed. CLSI supplement VET01S. The USA.
Daka D, Silassie SG, Yihdego D. 2012. Antibiotic-resistance Staphylococcus aureus isolated from cow’s milk in the Hawassa area, South Ethiopia. Ann. Clin. Microbiol. Antimicrob. 11(26): 1-6.
Devriese LA, Hommez J. 1975. Epidemiology of methicillin-resistant Staphylococcus aureus in dairy herds. Res. Vet. Sci. 19: 23 -7
[FDA] Food and Drug Administration. 2005. Isolation and identification of Staphylococcus aureus in foods. In Bacteriological analytical manual 8th ed. Gaithersbug: AOAC International.
Fessler A, Scott C, Kadlec K, Ehricht R, Monecke S, Schwarz S. 2010. Characterization of methicillin-resistant Staphylococcus aureus ST398 from cases of bovine mastitis. J. Antimicrob. Chem. 65(4): 619-625.
Kateete DP, Kabugo U, Baluku H, Nyakarahuka L, Kyobe S, Okee M, Najjuka CF, Joloba ML. 2013. Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda. PlosOne. 8(5): 1-12.
Khairullah AR, Ramandinianto SC, Effendi MH. 2020. A Review of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) on Bovine Mastitis. Sys. Rev. Pharm. 11(7): 172-183.
Klobongona MLMNB, Afiff U, Rotinsulu DA. 2019. Efektivitas antimikroba terhadap Pasteurella multocida dan Mannheimia haemolityca dari sapi yang diduga menderita bovine respiratory disease kompleks. ARSHI Vet. Lett. 3(2): 39-40.
Li JP, Zhou HJ, Yuan L, He T, Hu SH. 2009. Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China. J. Zhejiang Univ. Sci. B. 10(10): 753-760.
Meutia N, Rizalsyah, Saiful R, Mitha KS. 2016. Residu antibiotik dalam air susu segar yang berasal dari peternakan di wilayah Aceh Besar. JITV. 16(1): 1-5.
Nam HM, Lee AL, Jung SC, Kim MN, Jang GC, Wee SH, Lim SK. 2011. Antimicrobial susceptibility of Staphylococcus aureus and characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Korea. Foodborne Pathog. Dis. 8(2): 231-238.
Nurhayati IS, Martindah. 2015. Pengendalian mastitis subklinis melalui pemberian antibiotik saat periode kering pada sapi perah. Wartazoa. 25(2): 65-74.
Ruegg PL. 2017. A 100-year review: mastitis detection, management, and prevention. J. Dairy Sci. 100(12): 10381-10397.
Rotinsulu DA, Afiff U, Septiriyanti D. 2022. Resistansi Escherichia coli asal feses sapi di wilayah Bogor terhadap antimikrob. ARSHI Vet. Lett. 6(4): 75-76.
Saini V, McClure JT, Scholl DT, DeVries TJ, Barkema HW. 2012. Herd-level association between antimicrobial use and antimicrobial resistance in bovine mastitis Staphylococcus aureus isolates on Canadian dairy farms. J. Dairy Sci. 95(4): 1921-1929.
Sashidaran S, Prema B, Yoga LL. 2011. Antimicrobial drug resistance of Staphylococcus aureus in dairy products. Asian Pac. J. Trop. Biomed. 1(2): 130-132.
Tyasningsih W, Ramandinianto SC, Ansharieta R, Witaningrum AM, Permatasari DA, Wardhana DK, Effendi MH, Ugbo EN. 2022. Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia, Vet. World. 15(8): 2021-2028.
Wang K, Cha J, Liu K, Deng J, Yang B, Xu H, Wang J, Zhang L, Gu X, Huang C, Qu W. 2022. The prevalence of bovine mastitis-associated Staphylococcus aureus in China and its antimicrobial resistance rate: A meta-analysis. Front. Vet. Sci. 10(9): 1006676.
Diterbitkan
2023-03-07
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ROTINSULU, Dordia Anindita; AFIFF, Usamah; MAGHFIRA, Chairani Ridha.
Staphylococcus aureus Resistan terhadap Berbagai Antimikroba yang Diisolasi dari Susu Sapi di Indonesia.
Buletin Veteriner Udayana, [S.l.], p. 325-331, mar. 2023.
ISSN 2477-2712.
Tersedia pada: <https://ojs.unud.ac.id./index.php/buletinvet/article/view/98699>. Tanggal Akses: 21 apr. 2025
doi: https://doi.org/10.24843/bulvet.2023.v15.i02.p20.
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