Keragaman genetik masyarakat Pande Bangke Mawong di Desa Sumita, Kabupaten Gianyar, Bali berdasarkan penanda DNA mikrosatelit kromosom Y
Abstrak
The Pande Bangke Mawong community in Sumita Village is one of the large Pande clans in Bali. The origins of the Pande population in the village of Sumita are known to still be controversial. This study was conducted to determine the genetic diversity of the Pande Bangke Mawong community in Sumita Village based on Y chromosome microsatellite DNA markers. Samples were taken in Sumita Village and several villages in Bali as a comparison with the purposive sampling method. Stages of research included DNA extraction by the modified phenol-chloroform method, DNA amplification using microsatellite DNA markers with five types of primers namely DYS19, DYS390, DYS393, DYS395, and SRY. Electrophoresis of PCR products with done using Polyacrilamide Gel Electrophoresis (PAGE) 10%, visualization DNA by silver nitrate staining method, and DNA typing was determined by measuring the length of the DNA amplicon by measuring the distance of its migration to the gel plotted on semilog paper. Allele frequency and genetic variation data were analyzed using GenAlex 6.5 software. Kinship relationships were analyzed by the UPGMA method using MEGA 7 software. Eight alleles and three haplotypes were found in the Pande Bangke Mawong population in Sumita Village. In other Pande population, 12 alleles and six haplotypes were found. The genetic diversity value of the Pande population in Sumita Village was 0.035 ± 0.014, whereas in other Pande population it was 0.432 ± 0.112. The variety of alleles and haplotypes found in the Pande Bangke Mawong population indicate a microevolution process caused by genetic mutations. The kinship relationship between Pande in Sumita Village is the closest to Pande community in Tenganan Pegringsingan Village and Renon Village, which are known as one of the old villages in Bali.
##plugins.generic.usageStats.downloads##
Referensi
Campbell MC, Tishkoff SA. 2008. African Genetic Diversity: Implications for Human Demographic History, Modern Human Origins, and Complex Disease Mapping. Annual Review of Genomics and Human Genetics 9(1): 403–433.
Covarrubias M. 2013. Pulau Bali Temuan yang Menakjubkan. Udayana University Press: Denpasar.
Darmada NW, Sutama MG. 2001. Asal Usul Warga Pande di Bali. CV Bali Media: Denpasar.
Foster P, Kayser M, Meyer E, Roewer L, Pfeiffer H, Benkmann H, Brinkmann B. 1998. Phylogenetic Resolution of Complex Mutational Feautures at Y-STR DYS390 in Aboriginal Australians and Papuans. Molecular Biology Evolution 15(9): 1108-1114.
Guermonprez JF. 2012. Soroh Pande di Bali: Pembentukan Kasta dan Nilai Gelar. Alih Bahasa Paturusi SA dan Widyastuti. Udayana University Press: Denpasar.
Huthcinson F. 2006. DNA Band Size Semi-log Plotting. Science Education Partnership Cancer Research Center. (serial online). [cited 2018 Sep. 25]. Available from: URL: ttps://libguides.fredhutch.org/SEP
Junitha IK. 2004. Keragaman Genetik Masyarakat di Desa-desa Bali Aga Berdasarkan Analisis DNA dan Sidik Jari (disertasi). Sekolah Pascasarjana Institut Pertanian Bogor: Bogor.
Junitha IK. 2007. Penggunaan DNA Mikrosatelit untuk Penelusuran Kawitan pada Soroh-soroh Masyarakat Bali (Suatu Kajian Pustaka). Jurnal Biologi 11(2): 50-54.
Junitha IK, Sudirga SK. 2007. Variasi DNA Mikrosatelit Kromosom Y pada Masyarakat Bali Mula Terunyan. HAYATI Journal of Bioscience 14(2): 59-64.
Junitha IK, Sudirga SK, Wijana MS. 2009. Variasi Genetik DNA Mikrosatelit Kromosom –Y Soroh Pasek Kayu Selem di Bali. Hayati Journal of Biological Researchers 3A(1): 39-43.
Junitha IK, Alit IB. 2011. Ragam Alel Mikrosatellite DNA Autosom pada Masyarakat Bali Aga Desa Sembiran Kabupaten Buleleng Bali. Jurnal Biota 16(1): 63-39.
Junitha IK, Pharmawati M, Rosiana W. 2012. Genetic Divesity of Soroh Celagi (Pasek Catur Sanak Clan) Based on Y Cromosomal Microsatellite DNA. Poster Presentation on ”4th International Conferences on Bioscience and Biotechnology, Denpasar, Bali” 21st – 23rd September 2012. Procedings, ISBN: 978-602-7776-48-7:239-243.
Junitha IK, Watiniasih NL. 2014. Male Genetic Diversity of Siwa Brahmin Clan in Bali Based on –Y Cromosomal Microsatellite DNA. Journal of Biology, Agriculture and Healthcare 4(1): 30-35.
Junitha IK, Wijana MS. 2017. Identifikasi Alel dan Kekuatan Pembeda Empat Lokus DNA Mikrosatelit Kromosom –Y pada Masyarakat Klan Pande di Bali untuk Kepentingan Forensik. Indonesian Journal of Legal and Forensic Science 7: 1-8.
Junitha IK, Watiniasih NL, Puspitha NLPR. 2017. Profil Genetika DNA Mikrosatelit Kromosom –Y Masyarakat Laki-laki Soroh Kayuan Pasek Catur Sanak Bali Mula. Jurnal Kajian Bali 7(1): 57-66.
Korompis GC. 2015. Biostatistika untuk Keperawatan. Penerbit Buku Kedokteran EGC: Jakarta.
Kupfer DM, Jenkins M, Burian D, Canfield DV. 2008. Use of Alternative Primers for Gender Discrimination in Human Forensic Genotyping. Office of Aerospace Medicine Federal Aviation Administration: Washington.
Mahardika IW, Junitha IK, Narayani I. 2016. Identitas Genetik Keturunan Soroh Pande di Kecamatan Seririt, Buleleng Berdasarkan DNA Mikrosatelit kromosom Y. Indonesian Journal of Legal and Forensic Sciences 3(2): 23-31.
Parra E, Shrriver MD, Soemantri A, McGarvey ST, Hundrieser J, Saha N, Deka R. 1999. Analysis of Five Y-Specific Microsatellite Loci in Asian and Pasific Populations. Journal Anthropology 110(1): 1-16.
Peakall R, Smouse PE. 2012. GenAlEx 6.5: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research - an Update. Bioinformatics 28(19): 2537–2539.
Poerba YS, Martanti D. 2008. Keragaman Genetik Berdasarkan Marka Random Amplified Polymorphic DNA pada Amorphophallus muelleri Blume di Jawa. Biodiversitas 9(4): 245-249.
Sanderson SK. 2011. Makrososiologi Sebuah Pendekatan terhadap Realitas Sosiologi. PT Rajagrafindo Persada: Jakarta.
Septiasari NPS, Junitha IK, Wirasiti NN. 2017. Ragam Alel DNA Mitokondria Masyarakat Soroh Pande di Bali dengan Metode PCR-RFLP. Jurnal Metamorfosa 4(2): 210-217.
Starr C, Taggart R, Evers C, Starr L. 2009. Biologi Kesatuan dan Keragaman Makhluk Hidup. Edisi 12 Buku 1. Salemba Teknika: Jakarta.
Tegelström H. 1986. Mitochondrial DNA in Natural Population: An Improved Routine for Screening of Genetic Variation Based on Sensitive Silver Staining. Journal Electrophoresis 7: 226-229.
Tishkoff SA, Williams SM. 2002. Genetic Analysis of African Populations: Human Evolution and Complex Disease. Nature Review Genetics 3(8): 611-621.
Underhill PA, Passarino G, Lin AA, Marzuki S, Oefner PJ, Cavalli-Sforza LL, Chambers GK. 2001. Maori Origins, Y-Chromosome Haplotypes and Implications for Human History in the Pacific. Human Mutation 17(4): 271-280.
Wening S, Prasetyo AE, Rozziansha TAP, Susanto A. 2016. Optimasi Protokol Ekstraksi DNA Elaeidobius kamerunicus. J. Pen. Kelapa Sawit 24(2): 77-86.
Wulandari NPP, Junitha IK, Wirasiti NN. 2014. Penelitian Pendahuluan Variasi Genetik Masyarakat Soroh Pande Berdasarkan Penanda DNA Mikrosatelit Kromosom Y: Masyarakat Soroh Pande Desa Abiansemal, Badung. Jurnal Biologi 18(1): 5-9.
